Coding

Part:BBa_K2680539

Designed by: Stephanie Do   Group: iGEM18_William_and_Mary   (2018-10-15)


TlpA39

TlpA39 is derived from TlpA, a constitutively expressed protein, which binds to promoter pTlpA and blocks the start of transcription of genes downstream of pTlpA.1 This form is TlpA is mutated so that it's redepression occurs at a higher temperature than normal TlpA

References

1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None